The killing is the first in the Israeli-Palestinian conflict in almost two weeks.
Medics said they dragged the unconscious women out of their destroyed car and declared two dead at the scene, where a Palestinian car also appeared to be smashed on the side of the highway.
Palestinian witnesses say Israeli police, armed with batons, tear gas grenades and smoke bombs, burst into the Al Aqsa Mosque by force and beat the women and men worshipping there.
Yazan Omar Jamil Khasib, 23, who was killed by Israeli forces near Ramallah in occupied West Bank, becomes this year's 100th fatality of conflict rooted in decades of Israeli occupation of Palestine.
Israeli Finance Minister Bezalel Smotrich said he signed a double offset of the "terrorist funds" that the Palestinian Authority transfers to the "families of terrorists".
Blinken's long-planned visit to Israel has taken on a new urgency amid the spiralling violence.
A Palestinian gunman had killed seven people near a synagogue in occupied East Jerusalem on Friday.
The Jenin incident raises the toll of Palestinians killed this month in the West Bank to 17.
The Palestinian health ministry says Israeli import restrictions on medical equipment are putting people’s lives at risk.
UN approved last week a resolution requesting International Court of Justice render 'advisory opinion' on the Israeli occupation.
Israel’s National Security Minister Ben-Gvir entered the Al Aqsa Mosque complex early Tuesday, a day after he announced he was postponing the visit due to warnings of unrest.
At least 150 Palestinians have been killed so far this year in the occupied West Bank, according to the United Nations.
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