The extended quarantine has been placed on central Uganda's Mubende and Kassanda districts as the government continues efforts to control the spread of the deadly disease.
The directive to close schools two weeks before the end of term has come after the death of eight children from the contagious disease earlier this month.
Uganda has so far recorded a total of 135 confirmed cases and 53 deaths, according to the health ministry.
The outbreak began in September in a rural part of central Uganda and spread earlier this month to Kampala, a city of more than 1.6 million people.
At least 65 people have been infected and 27 killed by the outbreak of the Sudan variant of Ebola in Uganda, which had its last outbreak of the virus in 2019.
Uganda — which shares a porous border with the Democratic Republic of Congo — has experienced several Ebola outbreaks in the past, most recently in 2019, when at least five people died.
The new case of a 46-year-old woman, who died on August 15, comes weeks after the country declared its 14th outbreak over.
Tanzania's President has said the "strange" disease may have been caused by "growing interaction" between humans and wild animals as a result of environmental degradation.
Ebola is endemic to the Democratic Republic of Congo, where it was discovered near the Ebola river in 1976.
Officials in Democratic Republic of Congo DR reported a total of 11 cases, including six deaths, since the outbreak started in the North Kivu province’s Beni region on October 8.
Health officials in eastern Democratic Republic of Congo say three new cases of Ebola are reported in the country.
Officials find case of Marburg, a highly infectious disease that causes haemorrhagic fever, in southern Gueckedou prefecture.
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