As Madrid rejected Catalonia's referendum results on October 1 last year, the country's affluent autonomous region continues to hold on to its secessionist sentiment.
The move comes after a German court ruled that separatist Carles Puigdemont could only be extradited on charges related to misuse of public funds.
A German court decided to allow former Catalonian leader Carles Puigdemont's extradition to Spain for misuse of public funds. If extradited, he will not be tried for rebellion.
Quim Torra, a close ally of former leader Carles Puigdemont, fell short of being elected the new Catalan leader by two votes.
Catalonia's parliament will start debating on whether to appoint staunch independence supporter Quim Torra as regional president, after deposed leader Carles Puigdemont stepped aside from the running.
After meeting former leader Carles Puigdemont in Berlin, a spokesman for the Pro-independence group in the regional parliament said they wanted to install him by May 14.
The march was called by a platform set up in March and backed by the Catalan branches of Spain's two largest trade unions, the CCOO and the UGT, to "defend Catalan institutions" and "the rights and fundamental freedoms" of its citizens.
Former Catalan president Carles Puigdemont makes a renewed call for Spanish authorities to open dialogue over Catalonia's secession claim, a day after he was released from a German prison.
Puigdemont, granted bail after German judges ruled he could not be extradited on charges of "rebellion" brought by Spain also called for "the immediate release of all my colleagues in Spanish prisons".
German prosecutors are seeking to extradite separatist leader Carles Puigdemont to Spain, where he faces trial for his role in organising an independence referendum.
Catalonia's former leader Carles Puigdemont addressed the protesters in an audio message from a German prison. He was arrested travelling back to Belgium where he has been living in self-imposed exile.
Protesters blocked a main motorway through eastern Spain near the French border, another highway leading to the city of Lleida, a national road between Tarragona and Valencia and several roads in central Barcelona.
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